Journal: International Journal of Molecular Sciences

Article Title: Epigenetic Consequences of in Utero Exposure to Rosuvastatin: Alteration of Histone Methylation Patterns in Newborn Rat Brains

doi: 10.3390/ijms22073412

Figure Lengend Snippet: Quantitative Western blot analyses of histone methylation patterns in the brains of absolute control and vehicle-treated newborn rats. Protein contents from the absolute and vehicle-treated controls were determined and assayed on Western blots. Gel-loaded protein samples were separated by gel electrophoresis, transferred to nitrocellulose membranes, and assayed for immunoreactivity with antibodies that recognize core histones and histone methylations. Grayscale digital images of the immunoblots were scanned and processed under identical settings to allow comparisons between Western blots from different samples. Values indicate the means ± SEM from at least 5 separate experiments. Integrated optical density data were analyzed by ANOVA using SigmaPlot. No statistically significant differences were observed between the absolute and vehicle-control samples. Hence, we refer to vehicle-treated controls hereafter as simply “controls”, and further data presentation uses the vehicle-based controls as the reference point.

Article Snippet: All statistical comparisons were made using SigmaPlot software (v. 12.3, Systat Software, Inc., Chicago, IL, USA), and data were analyzed with one-way analysis of variance (ANOVA) or the Mann–Whitney rank–sum test.

Techniques: Western Blot, Methylation, Control, Nucleic Acid Electrophoresis